Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKQTLSYDEETRGKTVINHMGGVFLYNRPEQILEQYELEVKSISRGR-----DSYQCDTEQG-PKILREYRGS----KERAGFLADMLDHLSGQG-RTVETVMRTKEGEPFSVNEEETKYILYQAFPGAECDTKNRADMLSAVRELALLHQSAQNYEGSVPEFL---KSGQNNLLLLYEKRNRE--LNKVRNYIRAKKKKNDFEMMFAVWYPEYVKKAQETTDILKDLGIQEQLIGFCHGDYNQHNVIFSREGIAVVHFENFLYQESVGDLANFIRKMMEKNNWNAGLGMDLIRGYDRVRKLSPEELKYLYVYLAYPEKFWKIANRYYNSHKAWLSGRNIEKLEKVVAQEDAREQFLQMLFHFTV 3F7W Chain:A ((7-263)) ------------------------------ARVTELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD-----------DRTLAMEWVDERPPT---PEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAAD---RGAL-------TPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPA-AHGGHREADLAMLALFG-------LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPL-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 21893 18.32 90.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 18.32 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: