Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEVREYGKKSQPKIRYSQFDAEDIRKELLERYGMEMPVECRLFDYGMNDIYRIKTKKETYFLRISLAGLHTKKDYEEEMHILNILIQ-NGIKAAAPVKMRGMPGRQEDYVWELHAP-EGIRYAMLFTEAPNSPS---DDKESSACNLGSMLAGIHNISDREKFQV--SRPPIDMCQLVEKPLSNIRPFLADR---EQDFSFLKESAEKLAGYIKGKLPETAPVYGFCHGDVHSGNVFFA-G---DKPTLFDFDCMGYGWRSYDICVYAWNETFLDENYIEGSVWKKYLEGYESVRPLSAEEKETIPAFAALRQLWLMGLHADVMDRNAGCCWYQDDYFDSQIKMFRLWLDRCSL 6EF6 Chain:A ((27-349)) ---------------------DVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVV-----PARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGD-TPRWGRWQDAEGVGASETALLTRAQDLLHRKLEE-YGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD---PRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTHSRESATKAI-SYAAGSCALAERYLSSD--

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1524 -48862 -32.06 -159.68 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -32.06 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: