Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRRHMNRFTIEQVLDIAEVIHSRGSRKVWRIG-DYILKQTPYE----GVNNEVATLRFVQQH--TTIPVPVVYDEWLSPDRRFHYILESRIPGKSLKQ-CWGVLSRKGRERIARTVLKYMQELSQFRSDRLESISSNKLRLNNFVPKPYKVLLNIWRTDNDIFDNEYRPALLS-SGVDDGVILVLKNTMPPCQGQYTLTHGDLFTGNIIVDPVKEQVTGIIDWESAGFWPAWFQYARITLGAGGWDDEWKALLSRAMKATHLPPHSKHGRIWWDAVYELLDHPGSSLAREWIKEIIRYLAGENVSLNNYDGFGL 5IQC Chain:A ((27-257)) -------------KVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE--LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ-------------NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF-EGKKCLCHNDFSCNHLLLDGNN-RLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI--GTNFGEDIL-RMYGN---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 883 -30884 -34.98 -139.11 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -34.98 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: