Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRNKQKSKDYQSFLKNRHDKLNTSDDVLEKIVKNAVGYGIKDKQKIVEGEINEVYDITPTKGDNLIVRVSREEH----NRFEAEKWALDNCRN-AGVSVPQIILLDEVETDKEKLHICVENKIEGVSLQELIKQKVLSQSEIKDLTISAGEILSKIHSIKAKNFGEINS-------LGVGKFETWPEYMLEQ-NKNSDIVLEKAKNISI---DPKLIQQAIEILEKTQDMYMNVDSRLAHSDYGIKHMLIHEGKINGIIDFENCKSSDIAYDFSYWNYFGKNRPSVEWLMEGYKSNGNFGENFEARMHLVKIKIALSLLIYYSDAGHGFAKEITTNNLIEDITFFNYVK 3F7W Chain:A ((2-271)) -------------------------VNSVAARVTELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD--------DRTLAMEWVDERPPTP-------------EAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPY--LRRAADRGALTPGDVRLVEKVLDAL--DHLAGDPEPPARIHGDLWNGNVLWQDDG-AVVIDPA-AHGGHREADLAMLALFG--LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFG--------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1283 -11186 -8.72 -44.04 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -8.72 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: