Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRDAHERLTERFEDYRIVRKLRRVPPHEVYEASVDSRRAVYKGDTGPTGNAAVEGRVTALVGRETAVPVPETLRVGDDCYVTAWHPDAPAPDSSDEADEAWAAAAGRGLATLHTETEPLVDGCGPFRAADGGVRVETADDWHAAAVDHVRHRRSVLAEYGHADVADAAVEFLRDRPDAFDGAGDPVCCHGWWTPEHVAVEDGRVACVVDFEHATAAPGEWDYWRTVTPTFAACDEDTTAARRAFRRGYESVRPFSPGFERRRPYYGLLNGVYYLQSLYVQDRHGPAATAERAARFRARIFETISELDRGRLGRTAHPSRRVAFARHTPTSVMSRFDAVLFDLDGTLCENEQDVEHLYFGAFERAEVEPFGAPSDLWAVLEGP--------PTT----PDEFEYYREGFRTVAAEHGRAEAD-VSAVAREFVDGIDYGAVSLLGGAEAALADAGVGHRVGLMTNGPEYRQSVKLEALGLEDAFEVVVYAGDMERRKPHTDPFDRAVEALGVDASATLYVGNSLKYDVAGAREAGLPVAWYPDGDADP-G-------SYDPDYVVESLAELADVLDGTVE 3UMG Chain:E ((7-250)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RSPSTGRNVRAVLFDTFGTVVDWRTGIATAVADYAARHQLEVD--AVAFADRWRARYQPSMDAILSGAREFVTLDILHRENLDFVLRESGIDPTNHDSGELDELARAW--HVLTPWPDSVPGLTAIKAEYIIGPLSNGNTSLLLDMAKNAGIP--WDVIIGSDINRKYKPDPQAYLRTAQVLGLHPGEVMLAAAHN-GDLEAAHATGLATAFILRPVEHGPHQTDDLAPTGSWDISATDITDLAAQLRAGS-

General information: TITO was launched using: RESULT: Template: 3UMG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1135 -10608 -9.35 -47.57 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.62 3D Compatibility (PKB) : -9.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_3UMG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3umg-query.scw PDB file : Tito_Scwrl_3UMG.pdb: