Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPSMCRLMPVSFRIRIYHFLSSLGSRVYGSSCSLRVQQL---PFGVYLKTKSVEDHQALKIEFGALQLVRSQTQIPVPRPLDLLSDTETSYLLTTTLPGQRFGSYIDILSDHDLDLFTRDMQRYLTQLRSISRPRTPKYEIRNAIGGPYYDYRIIAAHDYDKECGDFFGPC--TDEEDFNNILRTPALPYVFHSTGHNIVFTHSDINMRNVLMHNG--RISGIADRENSGWFSDHWEYTKAHYVTKLHK---RWLAVVNRIFESFGDFTLDLEIERRLWEYCF 4H05 Chain:B ((22-247)) -----------------------------DGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAE-VGIPVPRVVEGGGDERVAWLVTEAVPGRPASARW---PREQRLDVAVALAGLARSLHALDWERCP---FDRSLAVTVPQAARAVAEGSVDL-EDLDEERKGWSGERLLAELE------RTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRG---------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 940 11515 12.25 53.31 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 12.25 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: