Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVEHILGEDWTVVPAGGSTGTAYYAKSD-QR-RLFLKRNSS---------PFLAVLSAQGI--VPKLVWTKRL-----ENGDVLTAQQWLDGKALNPEE-MQHPRVAKLLSKIHHSSELLDMLLRIEKQTQMPTQILAKMKEKVKT-----------T---DD----ADCSL----VIHKALKYLEDR----LPYVYYSKPVVCHCDLVHHNWMLSKNGELYLVDWDNAKVGDPAMDIGRILQSYIPKQ-----EWDQWLSHYGEE--NNAHFMQRMHWYLMID--AIHGYTWNVRRDKMKVAHQHMSELQQLLDQVNDWNL 4PDY Chain:A ((39-335)) -------------------HADGNIEVWTDSKGRRYAAKRSSIAPAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRSFRTDWK--------DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDAVRNIPKSEYRAVEAILRFPYLPWRIAHARYHF---AADPSQLDALQQYAVQAEKR--

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 947 13309 14.05 55.92 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 14.05 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: