Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIRR------SELAAQEVAYHNTDRSIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFET--------HKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKEEQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 4JXF Chain:A ((15-264)) ------------------------------------------LGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQL--KHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYL---------------------------------------------------------------------HSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHE---------------------------------SDVWSLGC-----MFYTLLIGRPPF----VKNTLNKV---VLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSV-LDHPFMSR---------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 839 -29586 -35.26 -148.67 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -35.26 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.182

(partial model without unconserved sides chains): PDB file : Tito_4JXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4jxf-query.scw PDB file : Tito_Scwrl_4JXF.pdb: