Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVVAALDRLAPRLSPT----ATRAINAR--RLSGGASLETWAFDLDD-------GAPLILRRRTSGSAL-RETALPLDHEASLIRAASA-VGAPAPSVVHVCAPEDDLGE-AYVMRRLEGETLGRRIVR-------DEAFAAVRPGLARRCGEVLARIHAVPTEGLPEL--ATSD---ARGELARYEAIYRELG---AERPIMEAAFRWLEAAAPSP--PERPVLVHGDFRNGNLMIHPKDGLVGVLDWELAHLGDPAEDLGWICVNSWRFGEWRKP--VGGF---GDYADLLAGYG--DNIPLERVRFWQALGSLKWGVMCLMMYQSFATGADRSVERAMIGRRASETEIDLVALMEAAA 3ATS Chain:A ((4-329)) -------AVISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT---DTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRD-FQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVELGDLHWFYVYSGVMWACVFMRTGARRVH------------------------------

General information: TITO was launched using: RESULT: Template: 3ATS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1226 -49215 -40.14 -175.14 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -40.14 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_3ATS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ats-query.scw PDB file : Tito_Scwrl_3ATS.pdb: