Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MSGGNMAEKDLATRVARVLGRNSVRLEDLPPGSSRGAARVLSEEGEPIAFLRIDLGVATDVTVGKALVSEMALVKRAHDLGFPVPKVFALLNDPPVAVMELAAGTARPDEAE---------AERVGPEFMGHIARLHSIAPAEFGLDAPAT--VSDAIRQDLDCY----------------MADAEARGITRRPLVRLAARILRHALPRSAEPPAMLHGDVGAGNFMVDNGRVSAILDWELAHAGDIHEDLAWLWTRGAHTAFGDPRQRIAEYEAAAGKALDPDRLAWHVAFVTFKSVIGLQRRMYAQGDDPGLLIILIAILTYETLLCAALGRILGIQLDMMEEAPVEGVTETVRLIELMRHVAPPANREGELLQQYLRAEAAQRPWNADRLRADARERLGAHPDQLDMLVDSAAPDRFADLLRVFGAAAGRRCKAFPNAERRVRRALAIGLGKQN 2OLC Chain:A ((5-396)) KTPLYETLNESSAVALAVKLGLTLTCQEI-----GDGNLNYVFHIYDRALIIKQAVPY--WPLTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTS--AETLIHGDLHTGSIFASEHE-TKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDG---------------------------------------------------------------------ADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKLLVK----

General information: TITO was launched using: RESULT: Template: 2OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1782 36179 20.30 104.26 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 20.30 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_2OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2olc-query.scw PDB file : Tito_Scwrl_2OLC.pdb: