Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSNNKLTRDELLSRFFPHYH-PVASLSQSGLSGGSVIISDG-DRRHVLRQPHDPSAATYHFRRQYHALRRLP-AR---LAPTPLFYSPCWLVVEYCAGEVKSE------------------LPASPQLSGLLYDLHQQPRFGWRV------S---LTPLLAQYWQRCDPA-RR-KPRWLRWHQRLRRQGEPRPLRLAPLHMDVHAGNIIHNE-SGL-RLIDWEYAGDGDIALELAAVWITPG--ERRRLVEAYARRAAIDAQQLWRQVARWRPWVLLLMAGWYEMRWRQSGDRQFITLADEIWCQLDNERKG 5UXA Chain:A ((2-298)) ---SKDIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDV-MPRTKVEKQALDLVNKYAISFQAPNWIIYTEELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDD-DMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFGEEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFAL----VSGIEEYEQMAKEALE--------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1077 -12587 -11.69 -48.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -11.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: