Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINEEVIWIVNSLVGE---FEIIKDHRNSSNRTGVLEINANNKRMFIKIHNRL--SRWSPEVYAYKNWTHILGEYAPRLIHSFNDDNFYGIITTPIYGKTVNEYQINDEDILEPIYYKAGELLKQLHNNFKGTYFGIPAID--GS-PLESKAKTDPVDYINS-ALEDILKSGYDKGLFNNSDKELVKWCMKHSDVFANSKPVPTNWDFSQNNWMVDEDGKFTGFIDFENMLWGIDVDSFGIVIERYTPNRPKLRKALFEGYGLENSEEKQLQLKIVSVKM--AIADITYGASVGNDRIFSLGRNLMDNLKMPGFKIH 3JR1 Chain:A ((20-309)) --MWKSISQVLAEQFGAYYFIKHKEKLYSGE-MNEIWLINDEVQTVFVKINERSYRSMFRAEADQLALLAKTNSINVPLVYGIGNSQGHSFLLLEALNKSKNK-----Q-----SSFTIFAEKIAQLHQIQGPDKYGLDFDTWLGPIYQPNDWQTSWAKFFSENRIGWQLQICKEKGLIFGNIDLIVQIVADTLSK-HNPKPSILHGNLWIENCIQVD-DKI-FVCNP-ACYWGDRECDIAFSSL--FE---PFPTNFYQRYNEIYPLEEGYLERKLIYQLYYLLNFSYRY-YNKKQSYVSLTQKLINQILHK-----

General information: TITO was launched using: RESULT: Template: 3JR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1384 57005 41.19 204.32 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 41.19 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_3JR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3jr1-query.scw PDB file : Tito_Scwrl_3JR1.pdb: