Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHPAAQPTNQTSSHSPASPSNTEPCEQQSLKQQLLKQQPLKEQSLKQQSREQQLHTWLNEVFPN-------QSLQHEKIPGDASFRSYHRLTVDN---------QHYIVMDAPPAKESVKEFIAVGNLMA-GYVHVPKMIATDEQQGFIVLEDLGNTDFADVIAKDLTDAGELAKTARYYQQAMQEILAIQKIDINDAKAVIPSYDDALLRREMGLFSDWFLPYI--GVKMTPDLETLWQDLQSDIIQQVI--AQPQVVVHRDFHSRNLMVLSH-----SDELGVIDFQDAVIGAYTYDLASLLRDAYIN----------------YDENWVNTHLAHYHQLAQLD----------KSLAEFTVDFNIMSMQRHLKVLGIFVRLFERDGKDRYLVNLPKVFNDLLTCLKAISQWDKRPTFDKFADWMTAAAAPAFQQKIQG 5FTG Chain:A ((9-346)) ---------------------------------------------------RRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNM-LFQCSLPDTTATLGDEPRKVLLRLYG---EAMVLESVMFAILAERSLG-PKLYGIFPQ--GRLEQFIPSRRLDTEELS----------LPDISAEIAEKMATFHGMKMPFNKE--PKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSI-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 963 -34175 -35.49 -130.94 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -35.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: