Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIKLGKKISEGKTVDVYESGD-LAVKVFKPDAPKTVVFYEAFMDSKVEET---GLNVPKIKEVELIDGKWAIATELIKGKTLAQIMQE--EPENCDGYLDDMVELQLSIHAKKVTGIS-----KLKDKLREEIESL----DVI---DHIKRYDLLTRLDS---TPKHVKLCHGNFGPENIVVTDDNK-VYIVDWIGASAGNASADVAKTYLKLALTS-TETAEKYLNLFCKKTNTAKTYVQEWLPIMAASTLAKGVTSKEEKDLLFTWLDVVDYS 5IQI Chain:C ((29-257)) ----DSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 789 37383 47.38 181.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : 47.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: