Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILTKLTQKDFEEILLRYSIGKYKAHEHISYALQNTVFI-LNTTK-GKYVLKIFEKSEADFVKFQIRITDYLYHKKVPVARIVKLKNGRNLLMYRNKLMAIQKFVGGNPAKKL-------NKELAGNLAKNLALMSKHLLKLKLKG-IFM--WSNDHEFETNTDIENIEKVNFVGYEKKLLEEIGKL-DRN-NLRRSVIHGDFHEMNLLTENN----ELRAIIDWDDAHEDYISYEIAVYLVSYFDAVSIKRSKEYIELFFKEFQRYIKLNEEEIKAVYYFMKQ-RLIGVIFWHQ--NQKKIHKNFEKRLDRSMKKLITRYVALENITLDEFMQLAERKSTSQKHHRAELKNKKNLYSVIVQD 3CSV Chain:A ((4-321)) ------SREDEIRDFLATHGY-ADWNRTP--------RYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPEIYAEEH--------ARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYA-ILG-DAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARR----DVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPR---VWAHFERGLAHPALASAAEEILNALP----------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 20000 14.96 69.20 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 14.96 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: