Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAAEMGMAETADLRTRLAPLFPGREISLRSAAAPVASPLHRAVASACVLAQ-PDGADPLFLKLAHPDMQDDLLDTDGAAARLAARAGVA-PALVLA----------RPGLLGFAYLALPWRYARTGDLQPPATLAAVLAAKKRLHEGDNLGRR---FCPFTRIAQLQAQAEA-------A--------QVPL----PAETAPLLRQAALIQQAL---------IASGMDLRFCHNHAAASNIMLNG-SQMMLVDFDIGGDNDPWYDVGALLNEVCD-----FDAPRRAAIEAYAGRC--DEALFARCRLYGAVDDLMWGLWGFTRAVTAPRDGIEFWKYGTWRLFQARTTMTMRDFELWLRTV 2Q83 Chain:A ((23-308)) --------------AENVLQGWDVQAEKIDV--------IQ----ALVWKVHTDS--GAVCLKRIHRPEK-K-ALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWI-EG-RPFEL---TVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPYE-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 893 -23768 -26.62 -102.89 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -26.62 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: