Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIRRSELAAQEVAYHNTDR-SIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFET------HKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKEEQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 6FD3 Chain:A ((30-282)) ------------------------------------------IGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFL---------------------------------------------------------------------HSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-MVGTPY---WMAPEVVT-----RKAYGPKVDIWSLGI-----MAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKEL-LQHPFLKLDKPLS----------------------------------------

General information: TITO was launched using: RESULT: Template: 6FD3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1179 40878 34.67 172.48 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 34.67 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.135

(partial model without unconserved sides chains): PDB file : Tito_6FD3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6fd3-query.scw PDB file : Tito_Scwrl_6FD3.pdb: