Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMRGGDLEAAALALWPDLARQMGEEAGLWRAAPLARREDARVARVLLRLDGPEGRRLVLKHQARPVVPDKFADGIAAHVAVQEVWPEGLPVLQAVDLERQACVMEYLPAR-PLSVLLEGAPLETQAALLRRAGAWMDGFHRALHGEARVFQPKFTIHFLRSVMGELRAGERRVAEPERFLVCAEALCAMQPDFEGQETVTAQTHGDLHQRNLVLDETRCWGVDFAGGRVVPVGHDIARLLTDYAILHAPKEAIPKGQVLPPQAQSAFFEGYGLVGPGD---PSVQLLLRNRVLAEWWGLPAREAERSRAQARRFGRLMALVDRVFG 3DXQ Chain:A ((4-262)) -------------DEARAKLAAIPMLAGYTGPLER----LGGLTNLVFRA-----GDLCLRIPGK---YINRANEAVAAREAAK-AGVS-PEVLHVDPATGVMVTRYIAGAQTMSPEKFK----TRPGSPARAGEAFRKLHGSGAVFPFRFEL---FAMIDDYLKVLS--NVTL--PAGYHDVVREAGGVRSALAAHPLPLAACHCDPLCENFLDTGERMWIVDWEYSGMNDPLWDLGDLSVEGKFN--------------ANQDEELMRAYFGGEARPAERGRVVIYKAMCDLLWTLWGLIQLA----------------------

General information: TITO was launched using: RESULT: Template: 3DXQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1168 -51989 -44.51 -208.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -44.51 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_3DXQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxq-query.scw PDB file : Tito_Scwrl_3DXQ.pdb: