Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVVAALDRLAPRLSPT----ATRAINAR--RLSGGASLETWAFDLDD-------GAPLILRRRTSGSAL-RETALPLDHEASLIRAASA-VGAPAPSVVHVCAPEDDLGE-AYVMRRLEGETLGRRIVR-------DEAFAAVRPGLARRCGEVLARIHAVPTEGLPEL--ATSD---ARGELARYEAIYRELG---AERPIMEAAFRWLEAAAPSP--PERPVLVHGDFRNGNLMIHPKDGLVGVLDWELAHLGDPAEDLGWICVNSWRFGEWRKP--VGGF---GDYADLLAGYG--DNIPLERVRFWQALGSLKWGVMCLMMYQSFATGADRSVERAMIGRRASETEIDLVALMEAAA 3ATT Chain:A ((4-329)) -------AVISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT---DTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRD-FQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVELGDLHWFYVYSGVMWACVFMRTGARRVH------------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1240 -48027 -38.73 -170.91 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -38.73 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: