Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDAQGSLVHELKSLPCWPAPVQAIRPL-TGLSGGSLLIELCD-GRRYVARSQNAQLQMQAVSRERERQVLCAIGEQVARSDFLPQVLFYNARWLVVSWLEGSPWPHTLADDACAQRHLVTLMRRFHPVDSE-LQ-L--------------DIPSRLQFYRQQLSAEK----Q---------PEALFSLC---ARFASLRSPCWWFPVLAHHDIHPGNVVSDG-ATLALIDWEYAARSPLACELILLFEANG---------FNRAQRQQFCRYYAEAIYSTETVYAADSKCVGSSAAALEDWLHRLQQDIASWQPWCEMLMAMWSAIRFEQTDDVQYAVWRDNYLKAAEQSLGKRE 2QG7 Chain:D ((109-454)) --------------------DSLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTD----NREREKKISCIL----YNKNIAKKIYVFFTNGRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDD-------------------EEL-INQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLPIF---

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1022 -13250 -12.96 -50.96 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -12.96 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: