Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----KLCKWTVL-NSEGEIIDSGSC--LNTEYMKHGDIHVEFENDCTPIIFGADRVAEKVTIKG--- 2KAF Chain:A ((1-67)) GSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDKIVYHTLESPVEFHLDGEVLSLDKLKSLLS

General information: TITO was launched using: RESULT: Template: 2KAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 188 3096 16.47 54.32 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 16.47 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_2KAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2kaf-query.scw PDB file : Tito_Scwrl_2KAF.pdb: