Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --AAAFGPLTRIDKPFTQYRITYTAPNKKPVVNKLG--YGNTAK--------LSVDGITYTAVADAKCKFVIVGSPWPAGHFTVGQV 1WI9 Chain:A ((98-169)) GSSGSSGFLTEF--------INYIKKSKVVLLEDLAFQMGLRTQDAINRIQDLLTEG-TLTGVIDDRGKFIYITPSGPSSG------

General information: TITO was launched using: RESULT: Template: 1WI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 1660 12.97 27.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 12.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_1WI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1wi9-query.scw PDB file : Tito_Scwrl_1WI9.pdb: