Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------METKLGRKIGEGGCSEVYEYGTEQPVKIIKLAKSNTSMDAMRIEYRNHRTTWELGLPVPQPFDL-------RVVDGRPGIVVEQIQGETLMQRFLRGLDQPEVSEDSPIYDDVRMTARILNALHSHSAEALPSQRESIKGALLGVESLTAPEKKEICAILDDLPVKQRLCHGDPNPGNIFVRHDEGLLI-DWMNASAGN------------------------------------------PEADLAEYILMVRYAVLPSHLGARIIGRFHSIREMMIDAFMEEYTRLSGVTAEEVDPWMLPMAARKLSADA---ISDEEKGLL-LHEIRVRLNAYKGAEESFRE 1B6C Chain:B ((14-339)) TTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSR-EERSWFREAEIYQT---VMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYT-----VTVEGMIKLALSTASGLAHLHMEIV---------------------------------GTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQ-KLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEG----

General information: TITO was launched using: RESULT: Template: 1B6C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1248 -18919 -15.16 -77.53 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -15.16 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_1B6C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1b6c-query.scw PDB file : Tito_Scwrl_1B6C.pdb: