Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILTKLTQKDFEEILLRYSIG-KYKAHEHISYALQNTVFILNTTKGKYVLKIFEKSE---ADFV-KFQIRITDYLYHKKVPVARIVKLKNGRNLLMYRNKLMAIQKFVGGNPAKK--LNKELAGNLAKNLALMSKHLLKLKLKGI-----FM-WSNDHEFETNTDI------ENIEK--VNFVGYEKKLLEEIGKLD--RN-NLRRSVIHGDFHEMNLLTEN--NELRAIIDWDDAHEDYISYEIAVYLVSYFDAVS--------IKRSKEYIELFFKEFQR--YIKLNEEEIKAVYYFMKQRLIGVIFWHQNQKKIHKNFEKRLDRSMKKLITRYVALENITLDEFMQLAERKSTSQKHHRAELKNKKNLYSVIVQD 4OCV Chain:A ((24-318)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHR-FEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNL----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1060 24570 23.18 94.86 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 23.18 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: