Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------PEHCFVRILKYEIPIHGDGYLVGSDVNWWVGNQKVDFHINNDCTIQRRGGTQEKK 1JEI Chain:A ((1-53)) DNYADLSDTELTTLLRRYNIP---HGPVVGSTRRLYEKK------IFEYETQRRRLSPPSSS

General information: TITO was launched using: RESULT: Template: 1JEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 111 8933 80.47 194.18 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 80.47 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_1JEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1jei-query.scw PDB file : Tito_Scwrl_1JEI.pdb: