Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PIPRII--GPCEVKVL------KAGVSQHLAYFKMGRETT---KTFFVPVPGGSTYLFELRTTYDCTATILRWTMPRDYG------ 1JJC Chain:B ((400-474)) P-PEAIPFRPEYANRLLGTSYPEAEQIAILKR--LGCRVEGEGPTYRVTPP---SHRLDLRLEEDLVEEVAR-----IQGYETIPL

General information: TITO was launched using: RESULT: Template: 1JJC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 179 -4467 -24.96 -77.02 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -24.96 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.071

(partial model without unconserved sides chains): PDB file : Tito_1JJC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1jjc-query.scw PDB file : Tito_Scwrl_1JJC.pdb: