Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEK-------YPGIQTRVLDVTKKKQIDQFANE----VERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARND------FLKRQKTGRFATAEEIAMLCVYLASDESAYVTGNPVIIDGGWSL 3LF2 Chain:C ((5-262)) --DLSEAVAVVTGGSSGIGLATVELLLEAGAAVAFCARDGERLRAAESALRQRFPGARLFASVCDVLDALQVRAFAEACERTLGCASILVNNAGQGRVSTFAETTDEAWSEELQLKFFSVIHPVRAFLPQLESRADAAIVCVNSLLAS-QPEPHMVATSAARAGVKNLVRSMAFEFAPKGVRVNGILIGLVESGQWRRRFEAREERELDWAQWTAQLARNKQIPLGRLGKPIEAARAILFLASPLSAYTTGSHIDVSGGLS-

General information: TITO was launched using: RESULT: Template: 3LF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1243 -23785 -19.13 -98.69 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -19.13 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_3LF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3lf2-query.scw PDB file : Tito_Scwrl_3LF2.pdb: