Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLGDNELTLTPI-PGKSGKAYMGSYPD--------GKRIFVKMNTSP----------ILPGLAREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPY----DMNRKQIVNILTRLHRSRPLMTQLSRLGYAMETPVDLLQSWQETAPDAL-------R-----KNHFISEVMADLRQTIPGFREDHATIVHGDVRHSNWIETDS------GLIYLVDWDSVRLTDRMFDVAHMLCHYIS-------------------EHQWKEWLTYYGYKY---------------NQTVLSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR 5FUT Chain:A ((30-351)) -----DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG--GAEAMVLESVMFAILAERSLGPKLYGIFP---Q--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEP------KWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES-TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKI---------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 764 4678 6.12 20.88 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.12 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: