Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTNPAAPAAPLAPPDPARIAKTVATKMPFRGDMTGL----TPLAGDASNRRYFRIALKGTPSALILMQLADPEGFKKSEEAVSGASAQIAELPFTNVLAHLQRT--GVTVPQLHYYDREAG----LLYLEDFGD---------------LTLAEACRDADHARLVALYRQAIDQLVLLQVKGTKPPAPGCIAFHRRFDV-PLLMWEFDHFLEYGIVARLGQPMKPADDKAVRAEFQRIAELLAG--QSQVFVHRDYHSRNLMVDGSRIGIIDFQDALM--GPATYDLASLLKDAYIELDDAVVDGLVDQFLDGLAAHGQGWADRAAFRRLFDFTS------------IQRNLKAAGRFVYIDRVKHNPKFLADIPRVLGYVKRNL--SKYPE-----------------------------LATLRQHLAPYVAELQ 1FP3 Chain:A ((1-402)) ----------------MEKERETLQAWKERVGQELDRVMAFWLEHSHDREHGGFFTCLGRDGR------VYDDLKYVWLQGRQVWMYCRLYRKLERFHRPELLDAAKAGGEFLLRHARVAPPEKKCAFVLTRD-GRPVKVQRSIFSECFYTMAMNELWRVTAEARYQSEAVDMMDQIVHWVREDPSGLGRPQLPGAVASESMAVPMMLLCLVEQLG-------EEDEELAGRYAQLGHWCARRILQHVQRDGQAVLENVSED---GEELSG---CLGRHQNPGHALEAGWFLLRHSSRSGDAKLRAHVIDTFLLLPFRSG-WDADHGGLFYFQDADGLCPTQLEWAMKLWWPHSEAMIAFLMGYSESGDPALLRLFYQVAEYTFRQFRDPEYGEWFGYLNREGKVALTIKGGPFKGCFHVPRCLAMCEEMLSALLSRLA

General information: TITO was launched using: RESULT: Template: 1FP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1512 -15462 -10.23 -47.00 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -10.23 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_1FP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1fp3-query.scw PDB file : Tito_Scwrl_1FP3.pdb: