Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQVQADRMILEDLLVSIQRDLGLVVHEAISIRRGWLNLKWKLETDQGTYLLKQYNRERFKLYDEEVLLMAFSQQIRLRRRGVK-CPKLLTNEGRYFLKSDQGERFMVMEFCEGQLVAPGG---------------------TNADQMRALGREVALMHRVLNDGSLGVKDKPQFVPPSRMERLDYWKRVMEQAAESEIPEVLTALETQYTATERFRDE-LNPMQPGWAHRDVWVDNLLFRPAGVAAILDFDRLNY-------DYPQLDIARAVLSCALHS-----------------------ELDVVLVSAFMEGYREEREVPKGYLVQGIRWLWYMESVWWVNANILEHQGPPARFAWEMDWLAKHLDMLPELLEQV 5JY7 Chain:L ((121-412)) -------------------------DAPPRVFGAEQSNTSVVF---GEDAIFKLFRRI------TPGVHPDIELNRVLARAGNPHVARLLGSFETEWE-GEPYALGMVTEFAANSAEGWDMATTSTRDLFAEGDLYAEEVGGDFAGEAYRLGEAVASVHACLAHELGTE-----EVPFP----ADVMAQRLAAAVD-AVPELREH---VPQIEERYHKLADTTMTVQRVHGDLHLGQVLRTPKG-WLLIDFEGEP-LDERRRPDTPVRDVAGILRSFEYAAHQRLVDQAGDDDDRARQLAARAREWVTRNCASFCDGYAAEAGTDPRDSADLLAAYELD-----------------------------------------

General information: TITO was launched using: RESULT: Template: 5JY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 910 17624 19.37 74.68 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain L : 0.61 3D Compatibility (PKB) : 19.37 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_5JY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5jy7-query.scw PDB file : Tito_Scwrl_5JY7.pdb: