Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSASPSLQVTAFEGNRCIASGPLASVAEKIKEVLARGEAASVLVFDDQTGSQVDLNLRTLSEAAQGMPTPLIAPEPLESRNAGRPRLGVVAREVTLLPRQWEWLGQQQGGASATLRKLVDQARRENAGEEKIRAAREALYRFMTAMAGNAPGYEEALRALFAGDVHRFSILTSDWATDVRKHVGKLAPAAFGYAPSPLDVVIPLTKREAVLRAFGTAEVQSIERITNGASGAGVFKVKAN-QVDYLLRIEGPPDGLRDPARQYACLKIAAEA-GV-APRLVYADAE-------SGVAVTDFIAPDSASVE---RSKADSLRRIVTAVRSLHDAPLFPELV---------DYLDGVDTLIRSCMETGILPKRAIETHRKFYGELAAAYPRKNLDLVSSHNDLNPGNVLFQK-E-RVWLVDWESAFAADRYVDLAAIANFFTTEENEKELVLESYFGAALN--DLHRARFFLMQQANRMFYAMVVLNFVAAAEPAIKLTAAGMKGIRWSEVRGEMARINTTETKVRFAGALLNEALLAFQSPRFSQAVAQVKGSSKRRGA 5IGI Chain:A ((7-283)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ADTSQLYALAARHGLKLHGPLTVNELGLD-YRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRW---LDDD-SSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG--EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALD-------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1083 -27412 -25.31 -109.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -25.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: