Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSALELPAKILEWIEKAAGSP-VLKADRIPGGGTRQGWLVDVAAAAAGDGSGKLFLRYSPERLPDRGAFHRLATEAEVLRALRGA-GVAVPAVCAVHPVE-------EAVLLERVPGETWFYRIRDPDEQVRVAQDFIRSLAKVHRLDPADLGVPGLGPVR--SAREHALERIAEIRRRGTAPDGSIDPLVRLSADWLESHVPDYDGPVVLVQGDTGPGNFLYQD-GRVTAVLDWELCHFGDPMDDIAWLSLRTVQDTFTHLPDRLAEYEKLSGHAID--VDRVRYYRVFAETTMATLTPNEGARAPAAEDGSGGADKDVGNHLLYAQLHRRLWLEALNAVMGLGLKKPVMPEPVDSSDWQPLYGDVLAMLRTITPRMGDALAVQWSKGIARLVRYLRELDACGRAGNELERSDIASLLGFTPLSLPAGRDALARANAAGRVSDVDFTRYLWNRVMRDDHLMRHASGALHSRTWPPLTNPS 5IGI Chain:A ((6-281)) ---TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDD------GRRWVLRIPRRAEV----SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV----VNDKRLHRWQRWLDDDSSW-PDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1168 -39164 -33.53 -150.05 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -33.53 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: