Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEYGWERTFPFLEIDNIIVNELFGEILEDKYITNISRINEGCRTTNYLIETNETDKKYILKIFFSSE----QNYIREIKLLNQLKENHIKNIPTVCKVSTHGSIENKEYAIYEYVEGKSIGKAISDGYVFDESLIIDVAKFLAQLHSYKFKKAGFLNE-----NLEIKEELPPLISWYEMF-MGDKAKQ-----RLGKKIVNQINEIVNQNQKILAELDNDIRLVHGDFQGTNILIRDNKLCGVIDWEFVMAGHPLADIGQFFRYEEYFNKDLIQVFKEEYNENSSYKLEDNWYKISKLRDSANLIQLINGYEDMPNKYSNIKKILINNINRLLD 3F7W Chain:A ((3-269)) ----------------NSVAARVTE-LTGREVAAVAERGHSHRWHLYRVELADGT-PLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD-------DRTLAMEWVDERPPTP----------EAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDH-LAGDPEPPARIHGDLWNGNVLWQDDG-AVVIDPA-AHGGHREADLAMLALF----GLPYLDRVRDAYN-EVA-PLAEGWRARIPLHQLHPLLVHVCL------------------------

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1261 -7877 -6.25 -31.26 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -6.25 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: