Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSIPSPSSCPARVDVPDALAALARFITLRLGCERVQIDACQRLSGGAIQENWLIRARVLNGGLQADRRWVLRT-DSPSSVAASMERSQEFAVLRAVHQAGVKVPEPLWLCQDATVLGRDFFLMQALGGSANGHRLSTDPELE-PMRKGLCHELGASLARLHGITPDHPELGFLPTPAPDP-VQASIDQYRHYLDALPGSHPVIEWGLRWCELNRPQPRPS-RLIHRDYRTGNYMVEAG----HLAGVLDWEFAGWGDPREDLGWFTARCWRFARPDREAGGVGALEDF-LGGYQEESG--YRPDRDELRYWQLMAH-LRWAVVALQQVQ-------RHLSGQQQSLELALTGRMVPELEQEILKLSGAPT--------- 3CSV Chain:A ((4-333)) -----------------SREDEIRDFLATHGYADWNRTPRYQRLR--------------------SPTGAKAVLMDWSPEEGGDT--QPFVDLAQYLRNLDISAPE-----IYAEEHARGLLLIEDLGDALFTEVINNDPAQEMP----LYRAAVDLLIHLH--DAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQ--------DARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1360 6120 4.50 20.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 4.50 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: