Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVEHILGEDWTVVPAGGSTGTAYYAKSDQRRLFLKRNSSP------------FLAVLSA-QGI-VPKLVWTKRL----------ENGDVLTAQQWLDGKALNPE---EMQHPRVAKLLSKIHHSSELLDMLLRIEKQTQMPTQILA---KMKEKVKTT-DDADCSLVIHKALKYLEDRLPY--VYYSKPVVCHCDLVHHNWMLSKN---GELYLVDWDNAKVGDPAMDIGRILQSYIPK----QEWDQWLSHYGEE--NNAHFMQRMHWYLMIDAIHGYTWNVRRDKMKVAHQHMSELQQLLDQVNDWNL 6EF6 Chain:A ((38-319)) -----YDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMG---------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 991 -15608 -15.75 -65.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -15.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: