Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------KLCKWT-----VLNSE-GEIIESGSCLNTEYMKHGDIH--VEFENDCTPIIFG---- 2KSW Chain:A ((1-66)) VPVGSDCEPKLCTMDLVPHCFLNPEKGIVVVHGGCALSKYKCQNPNHEKLGYTHECEEAIKNAPRP

General information: TITO was launched using: RESULT: Template: 2KSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 173 -2926 -16.91 -65.01 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -16.91 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.140

(partial model without unconserved sides chains): PDB file : Tito_2KSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ksw-query.scw PDB file : Tito_Scwrl_2KSW.pdb: