Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------RRVFKI-----DGEEFTPLETDCLIRIFKNKKTVHALFV--PPGT-ELDWRLDHN-HYAVGIKHNCDIIIGWKPSSWRIEGD----------------- 1QFH Chain:A ((750-857)) GANGEDSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEEITLDAIDNQDGTYTAAYSLVGNGRFSTGVKLNGKHIEG-SPFKQVLGNPGKKNPEVKSFTTTRTAN

General information: TITO was launched using: RESULT: Template: 1QFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 226 14377 63.61 199.67 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 63.61 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_1QFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1qfh-query.scw PDB file : Tito_Scwrl_1QFH.pdb: