Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FDCTIERRLGTQTKARA----RTSAKAMPEMIPAESGKYTVIAGTRVWVYGDCRTYPTHLPDGSTISGRAF--------------------------------------------------------------------------------------------------------------------------------------------- 4D7E Chain:A ((1-413)) METLLVVGAGPKALAVAAKSHVLRQLGLSAPRVIAVEAHAVGGNWLASGGWTDGRHRLGTSPEKDIGFPYHSTWARGHNREINEAMMAFSWTSFLVEHGTYAEWIDRGRPSPQHHVWAKYLQWVARKIDLELVLGKVRTIRQGWSVEVAGATTELEADGLMITGPGQSTKALAAHPRVLSIAEFWDLAGKRKLPISSRAAVIGGGETAGSALDELVRHEMLTISVISPMASYFENSLFSDPTKWNALSIQERRDVIRRTDRGVFSVRVQESLLGDNRVHHLQGRVTRIVGQGDGVAVTLRNEMRADQVHNFDLVVDATGGQPLWFLDLFDSESADLLELAVGGPLTQQRIESSIGYDLAVTGLGAKLYLPNMAALAQGPGFPNLSCLGELSDRVLR

General information: TITO was launched using: RESULT: Template: 4D7E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 178 3574 20.08 53.34 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : 20.08 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.220

(partial model without unconserved sides chains): PDB file : Tito_4D7E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4d7e-query.scw PDB file : Tito_Scwrl_4D7E.pdb: