Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFRSNNPITRDELLSRFFPQY-HPVTTFNSGLSGGSFLIEHQ-GQRFVVRQPHDPDAP-QSAFLRQYRALSQLP-A-CI-APKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRIT-------------LLPLLEL---------YWQQSDPAR-RTVGWLRMLKRLRKAR---EPRPLRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ 3F7W Chain:A ((3-287)) ------NSVA--ARVTELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWDDRTLAMEWVDERPP-TPEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPA-AHGGHREADLAMLALFGLPYLDRVRDAYNEVAP-LAEGWRARIPLHQLHPLLVHVCLFG--------AAYRTTLVDTARAALR--

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1270 -19897 -15.67 -78.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -15.67 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: