Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVETAYAEPPLAAWSSILTAPRAQRMAHLNPDVRLWSVTAD-DRSYIVRSFGRWRPGAGMVDEYRVLLHLKDA-GFPVAVPIVTDAGTLAARDGHRTYVLVPRLAAEKQEEL----PTGDVCFQIGSAIGNLHAALAQYPWPV--RSYEHDVLQQAFDEALQKLPQAV-RESSVRPHQ-AVVTSSL-RGLPRQLIHGDCNAGNILVSR-GRVSGVIDLDQLRAAMLAAE-ITLTSWSHVL----LTERRDR------ARPDERARYDAGVAALSWISRHFAELNAALA 5IGI Chain:A ((8-283)) --DTSQLYALAARHGLKLHGPLTVNELGLDY--RIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAE-LVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALD-------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1091 -42847 -39.27 -170.03 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -39.27 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.269

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: