Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKTHFSEHTLKDILSRYTL-GELLAFEPVTTGTVQTNYFLQTTQGRFVFRYYQNRSLESVLFESELIRYLTRCNYPCPA-------VLQDRNGEHAGIYNEKPYAIFEFA--TGQHLDHPNEAQKRQLVQKVAELNSITRGYKPLHTP------YRLNYNVESCWELAQEATQRINTAQAREKLAWLEHTLAGLHLPPSL-----PRGVCHCDFHFSNLLF--TDGKFSALIDFDDANYTFLLYDLAALINPFQASFDWNTWPRFQEEENV-FDFSETRTILQEYTRHR--ALSMDEKWHLFDVYKLSILFDCIWYFERWEGEDFYEQRKIAYLDRLGRDQFYAQIFAD------------------- 4OCV Chain:A ((24-378)) -------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPD---TVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDE----RDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPE-HNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1486 18976 12.77 61.21 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 12.77 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: