Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTERAEFDADAAEAILRRACAVADLDPDGIEILRLGDHAVFRIDG--G-RVISRVGRGADRLASVRREVAVARWLADKEYP-AARLVT-DAEQPIV-IEDHPVTFWEGLADGDT----YASTAEMGVLLRRLHELEAPPFSLP--RL-QPFDKVASRLER-----AVIPE------------STRAFLRSMARDLEAEYGR------L-EFSLPSGHLHGDFNVGNVLRDAAGHPRVIDLDGFATGPREWDLMQTAMYYDSFGWHTEAEYADFVVGYGFDVREWAGYAVLRSVRELLMVTWLSQNAGANPRAADEVQKRVATLRSGGSRRDWAPF 4PDY Chain:A ((22-283)) ---------------LIPQAVVSKYDLAIQQR---HADGN-IEVWTDSKGRRYAAKRSSIA--PAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRS-FRTDWK--DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDA--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 792 -14479 -18.28 -65.81 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -18.28 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: