Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVEHILGEDWTVVPA-GGSTGTAYYAKSD---------QRRLFLKRNSSP-----------FLAVLSAQGIVPKLVWTKRLENGDVLTAQQWLDGKALNPEEM----QHPRVAKLLSKIHHSSELLDMLLRIEKQTQMPTQILAKMKEKVKTT--D-----DAD-------CSLVIHKALKYLEDRLPYVYYSKPVVCHCDLVHHNWMLSKNGELYLVDWDNAKVGDPAMDIGRILQSYI-------------------PKQEWDQWLSHYGEENNA-----------HFMQRMHWYLMIDAIHGYTWNVRRDKMKVAHQHMSELQQLLDQVNDWNL 1NW1 Chain:B ((75-398)) ----------HLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHLGPKLYGIF---SG--GRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKE-----P-DYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHIS-LSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELS---------------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 862 -12870 -14.93 -57.20 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -14.93 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: