Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMRGGDLEAAALALWPDLARQMGEEAGLWRAAPLARREDARVARVLLRLDGPEGRRLVLKHQARPVVPDKFADGIAAHVAVQEVWPEGLPVLQAVDLE---RQ----ACVMEYLPARPLSVLLEGAPLETQAALLRRAGAWMDGFHRALHGEARVFQ-----PKFTIHFLRSVMGELRAGERRVAEPERFLVCAEALCAMQPDFEGQETVTAQTHGDLHQRNLVLDE--TRCWGVDFAGGRVVPVGHDIARLLTDYAILHAPKEAIPKGQVLPPQAQSAFFEGYGLVGPGDPSVQLLLRNRVLAEWWGLPAREAERSRAQARRFGRLMALVDRVFG 5IGI Chain:A ((6-255)) ---------TADTSQLYALAARHGLKL-HGPLTVN----ELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRR------EFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGE----------------EGLAKLLLTYEAAG------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 981 -52171 -53.18 -222.00 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -53.18 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: