Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------------------------------RPCRVDLYEFVHTKPGLTSWSWVHRFNGDARRASLFRAMGYLCSVDYNCKNFHCEKLTSWRW 1V8E Chain:A ((8-224)) PLRLGHRGAPLKAKENTLESFRLALEAGLDGVELDVWPTRDGVFAVRHDPDTPLGPVFQVDYADLKAQEPDLPRLEEVLALKEAFPQAVFNVELKSFPGLGEEAARRLAALLRGREGVWVSSFDPLALLALRKAAPGLPLGFLMAEDHSALLPCLGVEAVHPHHALVTEEAVAGWRKR-GLFVVAWT--VN-EEGEARRLLALG----LDGLIGDR-PEVLLPLGG

General information: TITO was launched using: RESULT: Template: 1V8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 174 -8570 -49.25 -161.69 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -49.25 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_1V8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1v8e-query.scw PDB file : Tito_Scwrl_1V8E.pdb: