Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---RICKVGIYRAAKDNNWRLRAA-RKIRISMDNTPMQFQWDYKDGASKEATYKV--ELHYEGCEAKVVGDQKLPAYLTIKGEI- 1FNM Chain:A ((404-482)) VPEPVIDVAIEPKTKADQEKLSQALARLA---EEDP-TFRVS-THPETGQTIISGMGELHLEIIVDRLKREFKVDANVG-KPQVA

General information: TITO was launched using: RESULT: Template: 1FNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 256 30729 120.03 426.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 120.03 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_1FNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1fnm-query.scw PDB file : Tito_Scwrl_1FNM.pdb: