Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GWCKVKVLYRYNSKEAQATYAEIDKETHWNLDGFPIVIK-PDGDCTVELVSGWVDHKTYFFKGE------------ 1E2W Chain:A ((169-232)) --------TIYNASAA-GKIVAITALSEKK-GGFEVSIEKANGEVVVDKIPAGPDLIVK--EGQTVQADQPLTNNP

General information: TITO was launched using: RESULT: Template: 1E2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 164 -3557 -21.69 -69.75 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -21.69 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_1E2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1e2w-query.scw PDB file : Tito_Scwrl_1E2W.pdb: