Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVEQNEFLSQVQLQILAEQAIKQYPSDY-QGQVKLLCQS-ENATYQVSTGSA-RYALRIHRPNYHSKLDIQSELEWLDALNA-SGIQVPVAIADQSGERVITLKLSNDIYRYAVLFNWVEGDMPTVEV------------DPTAFEQLGQITAKLHAHSKTWQAPENFQRIVWNHETMVGADGHWGNWKNAPHLRPQDHGVIEEAIAQISKDLNFFGKTQERYGLIHADLRLTNLLLQQE-RIGVIDFDDCGMSWFMHDLAAAISFNEHYANAPHWVDYWLKGYERVGHIQSEEYEMIPTFIMQRRIQMMAWNGSHAQTEMAQSLGDQWSNETVRLCKKYLNGQMPVGI 5UXD Chain:A ((9-255)) ----------------ALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDV-LERAAVEGRLLAMLAPHLDVAVPDWRIST---------------SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIE--VRSPAQVRG---AWRQDLAR--VGAE-FEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVS-----APSAIFEVALQAYAEGGGRP----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 916 -17954 -19.60 -78.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -19.60 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: