Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAGCDRCRASSPPDPVNESAEFLQLLRQDGVVRTDDARLMPLTGGVSCEIYLVED-GAERFVVKRALAKLKVKADWFADICRNRYEWEFIRYVSRFLPAVVPALRHCS-------ATGNYFAMEYLGGEFQNWKQQLLAGEARPEAAAQAGGLLAEIHRHSTGDAEAKR-IFDTTPNFY-QLR-IEAYLLATGVKH-PELR-PLFEAEAARL-----AATRECLVHGDFSPKNILVSPERMVLLDCEVAWYGDPSFDLAFMLNHFFLKTLLHAPREVGMRRMVEAFWAAYQTGRPTPELETRVGRLLVMLLLARVDGKSPVEYLDVGRQDFVRQFARVMLHEEAPSLGVVTNAWFARLSQFTS 1ZYL Chain:A ((12-257)) ------------------HPDTIMDALFEHGIR--VDSGLTPL-NSYENRVYQFQDEDRRRFVVKFYRPERW-------TADQILEEHQFALQLVND-EVPVAAPVAFNGQTLLNHQGFYFAVFPSVGG--RQFEAD------NIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDA-TLIPSGLKAAFLKATDE-LIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKAE----------QRMQLETIIEAYEEFSE--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 917 -42112 -45.92 -184.70 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -45.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: