Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINEEVIWIVNSLVGEFEIIKDHRNSSNRTGVLEINANNKRMFIKIHNRLSRWSPEVYAYKNWTHILGEYAPRLIHSFND-DNFYGIITTPIYGKTVNEYQINDEDILEPIYYKAGELLKQLHNNFKGT--YFGIPAIDGSPLESKAKTDPVDYINSALEDILKSGYDK--GLFNNSDKELVKWCMKHSDVFANSKPVPTNWDFSQNNWMVDEDGKFTGFIDFENMLWGIDVDSFGIVIERYTPNRPKLRKALFEGYGLENSEEKQLQLKIVSVKMAIADITYGASVGNDRIFSLGRNLMDNLKMPGFKIH 3W0S Chain:A ((7-234)) --TATSVEKFLIEKFDSVSDLMQLSEGE-ESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASA-ALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL--PE-TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQG----IGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAED-CPEVRHLVHADFGSNNVLTDN-GRITAVIDWSEAMFGDSQYEVANIFF-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 940 -28561 -30.38 -128.08 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -30.38 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_3W0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0s-query.scw PDB file : Tito_Scwrl_3W0S.pdb: